DNA Extraction by Phenol Chloroform

This is cool!!!!!

I want to doing this experience

Excellent,thanks a mill

not sure if you already found out. IIRC phenol is a bit soluble in water. chloroform prevents that. and isoamyl alcohol prevents bubbling, which i'm not so sure what that will affect. perhaps visibility. using phenol n chloroform alone would do in ratio 1:1. repetition needed to make sure you have removed as much as protein. usually people do 3 cycles of purification before precipitating DNA

Very good, deep thanks

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Thanks a lot.

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Thank you for this video, it is so nice. But I confuse is this genomic DNA or plasmid isolation and how to make lysis buffer?

A very good movie, however, all mixing steps were performed by pipetting and this could be problematic for DNA sharing or damage as we know that gDNA is a fragile component. I prefer the up side down method to avoid such sharing.

can we change the ratio of PCI????

I have a little question. Why are you transferring Phenol:Chloroform:Isoamyl in an open area. This solution is toxic and carcinogenic so it must be done under fumehood. Thanks.

Is it a technique for any microorganism's DNA isolation???

what is the function of Proteinase K in this experiment?

Very poor

Sry..Its really amazing

I've tried to extract s.aureus dna from wound samples for more than 3 times but my test is negative😢 whats the reason behind failure

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Good job!

To remove the last bit of ethanol...just keep the vial open..it will evaporate....it's risky to pipet it out,your DNA may come out in the pipet...

Another thing....70% ethanol will not work(as explained in the video) and use chilled ethanol 99.9% for better result...

Why does the DNA sticks to the side wall of eppendorf tube at the last step of centrifugation ?

Wow, that was very tedious

What is the purpose of adding chloroform into the last step?

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Great video, you may use guanidine thiocyanate is used for RNA isolation and can solubilize proteins.

One of the best tutorial.thanks
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Thank you so much! This video remains super helpful after 10 years!

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Had to dislike!!!! Not even using safe-lock tubes??? and where the fuck is the fumehood??? This is shit-tier DNA purification. And why would you even spin for 10 minutes? Just do 5 at 16000 rpm you fucking scrub! omg. AND MIXING BY PIPETTING? what is this, the fucking stone age? We have vortexers ffs. Omg... 5/7. Garbage

Great video... It helps me a lot

hey can you please recommend me 10 books on this, im doing practical and google is not helpong

Can I ask something? What is the lysis buffer recipe and can the DNA from this protocol be used for PCR with the precise result?

People still do this?

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You should also mention the reasons that why,for what purpose we use particular chemicals,.. Otherwise great tutorial.

What is the temperature for centrifugation?

How much volume do you use to resuspend DNA in TE buffer?

Thank you 🙏